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KMID : 0377619970620040359
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Korean Jungang Medical Journal
1997 Volume.62 No. 4 p.359 ~ p.364
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Cloning and Expression of the Gene that is Responsible for the Uninducible β-lactam Resistance in MRSA
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Abstract
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Methicillin-Resistant Staphylococcus aureus(MRSA) isolated clinically from hospitals has a characteristics of highly resistant to not only β-lactam antibiotics such as penicillin, but also amino glycosides, macrolides which have differences in the mechanism of targets. All of the MRSA strains examined so far contain the MRSA-PBP gene(mecA) of 2,010 base pairs(bp) in size and its gene product-MRSA-PBP(Mw.76,462), a novel penicillin-binding protein (PBP)-2´ or 2A that has very low affinities to the most β-lactam antibiotics. In most of MRSA strains such as TK784, MRSA-PBP produced inducible by contact of the cells with β-lactams. Expression of mecA is regulated by some regulatory genes such as mecRi and mecI1011´1, which are located upstream of the MRSA-PBP structural gene in the opposite direction. However, some strains such as TK731 produced constitutively MIRSAPBP, whether β-lactams is being or not.
In this report, we amplified the interested DNA fragments contained MRSA-PBP gene and its upstream region by polymerase chain reaction(PCR) and sub cloned into E. coli plasmid vector and confirmed the expression of MRS-PBP in E. coli to study the uneducable mechanism of MRSA-PBP production in MRSA strains at the molecular level.
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